-Custom Services of Protein Expression by the pin-point labeling-   [Flowchart of Custom Services] Please confirm the flow of custom services here. [Outline]   Using pin-point Protein Labeling technology, ProteinExpress can synthesize proteins containing unnatural amino acids at any desired position. We can provide custom services from artificial gene synthesis to protein purification for pin-point Protein Labeling. Flowchart of Custom Service of Protein Expression by pin-point labeling [Contents of Service] Synthesis of the Unnatural Aminoacyl-tRNA Requested unnatural aminoacyl-tRNA are synthesized Preparation of Template  The expression template (plasmid or PCR product) is constructed after receiving the cDNA from customers.  If the customer does not have the cDNA, it is possible to obtain the cDNA through our custom gene synthesis service. Trial Expression Confirmation of protein expression (soluble or insoluble) by small scale (50 µL) is conducted Optimization Conditions of protein expression (additive surfactants or chaperone) are optimized. Scale-up The scale is adjusted to your desired scale. Purification The target protein is purified. Contents of the Service Synthesis of unnatural aminoacyl-tRNA   Synthesis of unnatural aminoacyl-tRNA Preparation of the template CS501 Linear template synthesis (Pin-point) CS502 Plasmid Construction (Pin-point) CS503 Plasmid preparation by large scale Trial expression CS504 Expression (Pin-point), 50µl Scale-up CS505 Expression (Pin-point), 1ml CS506 Expression (Pin-point), 10ml Purification CS507 His column, 1mL (Pin-point) CS508 His column, 5mL (Pin-point) CS508 His Spin column [Related pages] CloverDirect TM tRNA Reagent for Site-Directed Protein Labeling Custom Synthesis of Unnatural Aminoacyl-tRNA Artificial Gene Synthesis Service (Sloning BioTechnology GmbH) Custom services of the protein production [References] 1) FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids Daisuke Kajihara, Ryoji Abe, Issei Iijima, Chie Komiyama, Masahiko Sisido, Takahiro Hohsaka Nature Methods, 3, 923-929 (2006). 2) Efficient Incorporation of Nonnatural Amino Acids with Large Aromatic Groups into Streptavidin in In Vitro Protein Synthesizing Systems Takahiro Hohsaka, Daisuke Kajihara, Yuki Ashizuka, Hiroshi Murakami, and Masahiko Sisido J. Am. Chem. Soc., 121, 34-40 (1999). [Questions about Products] >>Online question Back to HOME Back to Custom Services Groups Back to "Pin-point Protein Labeling" page