Custom service of the protein expression (mammalian cell)
<<OUTLINE>>
·The target protein is expressed transiently by mammalian cell (HEK293 and
COS-7, etc.).
·We can construct the expression vectors even when you don't have
your desired gene.
·Please inquire if you don't have the cDNA.
·We are able to optimize the expression condition, to culture by large-scale and to purify the target protein.
·Please inquire about the establishment of a stable cell line.
<<Contents of Service>>
| Preparation the Template |
The expression vector is constructed after receiving the cDNA from customers.
If you don't have the cDNA, we can obtain the cDNA by our custom gene synthesis
service. |
 |
| Trial Expression |
Productivity and solubility of the target protein are confirmed by small
scale. |
|
| Scale-up |
The scale can be adjusted your desired amount. |
|
| Purification |
The target protein is purified. |
<<Examples of Protein Expression>>
<example 1>
Expression of fluorescent protein in mammalian cells (four transfection
conditions)
A; Detection and quantification of the expressed protein
B; Phase-contrast and fluorescent microscopic analysis
<example 2>
Expression of GPCR fused to fluorescent protein (three transfection conditions)
green; GPCR fused to fluorescent protein
blue; cellular nucleus
<example 3>
Expression of two secretory proteins (four transfection conditions)
Western blot analysis of the secreted proteins.
|
Contents of Service |
| Preparation of the template |
| CS701 |
Plasmid construction (mammalian cell) |
| Trial expression |
| CS702 |
Expression (mammalian cell) |
In case of scale-up expression, we will present the price after the trial expression service.
Technical Inquiry
Return to Products & Services
Return to HOME |
