·The target protein is expressed by the Brevibacillus choshinensis (brevis) expression system.

·We can construct the expression vectors even when you don't have your desired gene.

·We can easily purify the protein because the recombinant protein is secreted into the culture medium.

·We are able to optimize the expression conditions, to culture large-scale and to purify the target protein.

Construction of the Expression Vector	The expression vector is constructed after receiving the cDNA from customers. If you don't have the cDNA, we can obtain the cDNA by our custom gene synthesis service.
Trial Expression	Productivity and solubility of the target protein are confirmed by small scale.
Optimization	Conditions of the protein expression are optimized (medium, cultivation time and so on).
Scale-up	The scale can be adjusted your desired amount.
Purification	The target protein is purified.

	Contents of Service
Plasmid Construction
CS201	Plasmid Construction (brevis)
Trial expression
CS202	Expression (brevis)
Scale-up
CS203	Expression (brevis), 100ml
CS204	Expression (brevis), 1200ml
CS205	Expression (brevis), 4800ml
CS206	Expression (brevis), 20L
Purification
CS207	Metal-Affinity column, 1mL (brevis)
CS208	Metal-Affinity column, 5mL (brevis)